22 Questions 19 Answers 0 Followers
Questions related from Sasidharan S Lucky
I used an antibody against H2-Kb SIINFEKL tagged with APC on Dendritic cells treated with nanocarriers carrying SIINFEKL peptide. After 3 hour incubation, the cells were washed 3 times with PBS...
11 February 2023 6,827 1 View
We have a patient derived xenograft model of H&N cancer. The primary tumor was HPV-16 positive. The early passages also showed HPV-16 positivity. However the later passages are not....
01 July 2020 5,921 0 View
I want to measure the number of cells that died following addition of Tcells to the cancer cell spheroids with and without checkpoint inhibitors. I prefer a 96 well plate format. Since both the...
26 February 2020 574 4 View
I am just curious to know what will happen if Tcells and cancer cells that are not HLA matched are co-culture together? Will the Cancer cells die due to allo reaction (GVHD) or will there be no...
28 January 2020 3,156 2 View
I have determined the alpha/beta ratio of the cells I am working with at hypoxic (a/b Hypoxia) by growing the cells at 1% hypoxic environment and normoxic conditions (a/b Normoxia) (21% oxygen)....
27 August 2019 2,682 2 View
I am trying to plot a radiation dose response curve using graphpad prism. I an using a nonlinear regression (curve fit)> linear quadratic survival (Y is percentage). X is radiation dose in...
02 January 2019 7,080 15 View
I have a mix of cd8+ and cd4+ tumor infiltrating t-cells isolated from patient tumor. I am expanding them in vitro in media supplemented with IL-2 at a concentration 5U/ml. I believe that IL-2...
16 April 2018 6,776 2 View
I am establishing a Tcell co-cultured 3D human cancer spheroid model. 1 hour after addition of HLA-matched Tcells to spheroids, I see that the Tcells infiltrate the spheroids. I used this model to...
10 April 2018 8,994 4 View
I am trying to compare and calculate the transport of my drug loaded nanoparticles and free drug using a caco2 cell transwell assay. I have 2 issues: 1. The drug concentration at 0 hours in the...
23 November 2016 7,611 8 View
I work with a 2 similar but structurally different PEGylated polymeric nanoparticles encapsulating a traditional chemotherapeutic drug. The nanoparticles are developed to be administered orally....
09 August 2016 9,931 9 View
My 26 aminoacid peptide, known to immediately trigger pore formation on the plasma membrane of the cells leading to more than 90% cell death (confirmed by both MTS and LDH assay) following its...
15 December 2015 7,095 8 View
My peptide is 26 amino acids long (mol wt : around 2850 g/mol), and is known to be a cell-penetrating peptide, I want to track its localisation in cell by labelling it with FITC. I am thinking of...
05 November 2015 7,020 5 View
My peptide is 26 amino acids long (mol wt : around 2850 g/mol), largely hydrophobic but soluble in water (>250mg/ml), and is known to be a cell-penetrating peptide. I want to track its...
05 November 2015 3,549 1 View
Is it possible to accurately measure the size of nanoparticles in RPMI with 10% FBS by DLS? Could DLS measurement be disturbed by the scattering light from serum proteins.
03 October 2014 2,078 6 View
I would like to check if the nanoparticles I am working with induce thrombogenicity, platelet aggregation or complement activation following its systemic administration in vivo (mouse model). I am...
06 June 2014 9,167 11 View
I am working with 50 nm titanium dioxide coated heavy-metal based nanoparticles. The size is 50 nm. 4 hours following IV administration of about 10 mg/kg (around 90-100ul depending on weight of...
21 April 2014 1,856 1 View
The nanoparticles I am working on aggregates in physiological media and conditions. In-vivo they seem to accumulate in the liver and spleen, and not in the target site (tumor). I want to compare...
07 January 2014 9,438 1 View
I am studying the biodistribution of Pegylated titanium dioxide nanoparticle in balb/c nude mice xenograft model. I inject 200 ul of 50 mg/kg nanoparticle suspension (suspended in PBS, and...
19 September 2013 692 32 View
I don't think that serum proteins are attaching to my nanoparticles when I incubate them in a culture medium with serum during in-vitro studies. I want to isolate the proteins attaching to the...
25 January 2013 6,969 3 View
Pegylation is a well established procedure. But is it important to optimize pegylation using a different molecular weight PEG at different concentrations to get the maximum coverage for a...
25 January 2013 3,768 12 View
I am trying to quantify the PEG density, coverage and finally the conformation of PEG grafted on a metallic nanoparticle coated with a thin layer of Silica. The PEG I am using is a Silane-PEG-NHS....
19 January 2013 1,812 1 View
I am working with NIR excitable nanoparticles on in-vivo tumor model. Does anyone know a safe laser power/ dose at the NIR (980 nm) range, that does not cause skin burn in mouse. What is the...
19 January 2013 1,012 3 View
