I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

No mRNA increase in PCR after successful transfection in ARPE-19 cells ?

Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...

18 February 2021 3,811 7 View

Do there exist stand-alone permanently in-ground soil-moisture sensors with an installed GSM module, for outdoor use?

Am searching for a stand-alone permanently in-ground soil-moisture sensor with an installed GSM module, for outdoor use. The idea is for the sensor to send a moisture reading via a cellphone text...

04 February 2021 2,047 4 View

What is the easiest way to remove the water from organic solvents?

Hello everyone! I have one question. I have couple of solvents and all of them were pure when they were bought but after some months or years of storage after opening I am not 100% sure about...

03 February 2021 2,154 8 View

Virus neutralization assay in BSL2 lab?

Hi I want to set up a virus neutralisation assay such as PRNT or FRNT at my lab (BSL2), but im uncertain of the bio safety requirements that has to be fulfilled in order to work with...

01 February 2021 5,626 3 View

Using LINK11 to Apply force in ANSYS APDL?

I'm trying to use LINK11 to apply a force as if it was a cable pulling a structure twowards a point in ANSYS APDL. When I solve the model, the deformation of the overall structure is far too...

30 January 2021 7,919 1 View

Cytoflex Flowcytometer, clotting issues and cleaning procedures?

We have been having clotting issues with our Cytoflex S from Beckman Coulter. Back in December 2020, we had to have the tubing system and sample needles replaced, but we are beginning to observe...

28 January 2021 6,897 2 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

What excamples do you know wasting unused contents of "empty" packaged products?

Most of us use tooth paste more times a day. The empty tube we throw away. I wondered how empty the tube really is. This does not sound being Research? I began to collect empty tubes...

19 January 2021 808 3 View

What is the largest disadvantage in using of face masks at present? What would you like to be improved?

We all use face masks many hours a day. I wonder what are your items you like to be improved. We have the opportunity to write an application for support to a sponsor. Your answers will help us a...

18 January 2021 1,311 4 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Why does the testosterone dissolved in dmso and methanol turns into a white dispersed precipitate when subjected to serum free media?

1mg of testosterone is dissolved in 20 microlitre of DMSO and Methanol respectively. The interpretation showed a positive result. However, when 1 ml of serum free media is subjected to the...

27 January 2021 8,213 1 View

Is there a formula or standard protocol for steroid hormones treatment in mice or rats?

hi, I'm going to determine the dosage of testosterone in order to inject to rats or mice subcutaneously. I wonder that is there a particular dose or multiple doses for that or not. thanks.

05 January 2021 5,921 2 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Analysis of significance by using R programming ?

Dear All, I have the data of metabolic pathways in case and control, well there are pathways which are represented several times due to the involvement of reactions in case, well now I would...

09 December 2020 4,494 5 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View

Why do cell sensitivity to antibiotic resistance increase after lentiviral infection?

03 November 2020 7,754 2 View

What is the difference between endometriosis and ovarian cancer in terms of signaling pathway?

Hello Recently, I am curious to know about mechanisms of endometriosis and signaling pathways. I have a question about the difference between signaling pathways in benign tumors and malignant...

26 October 2020 3,891 6 View

Hormone levels in male mice ?

Dear Colleagues, Can anyone tell me if there are Data about hormone levels in male mice , E2 Estrogen, and Testosterone blood levels in male mice. Thank you very much.

20 September 2020 1,035 3 View