I work with both lines, but it is very difficult to expand them under standard conditions.
I work with human embryonic stem cells ... what difficulties are you having?
Thanks, but the HESC cells have a very slow growth under normal conditions (DMEM HAM/F12 medium with 10% FBS)
In my experience, hESCs take about a week to go from one passage to the next. Their slow cell cycle is part and parcel of their stem cell character.
In general, FBS is not normally involved in stem cell culture. It is recommended against, since it encourages differentiation into non stem cell lineages. To maintain stem characteristics, you need a serum-free media such as "mTeSR-1" from Stem Cell Technologies or "Essential 8" from Life Technologies.
But even so, I would expect slow growth to be a sign that it's working, not a problem.
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