Maria Lorena Oróstica

4 Questions 5 Answers 0 Followers

Questions related from Maria Lorena Oróstica

Nitrocellulose Membrane capacity?

Hi, Do you know how many proteins (ug) can I use in a Nitrocellulose Membrane of 0.45um (300mm x 4m) Amershamm Protran GE? 80ug? 100ug? Thanks!

17 October 2018 6,227 0 View

Can Orthovanadate increases STAT3 expression and activation?

Hi, I need to know if Orthovanadate (sodium) can induce expression of STAT3 or increases its phosphorylated form (active STAT3 form). The use of Orthovanadate in lysis buffer to obtained total...

14 October 2018 3,988 2 View

MiR controls in qRT-PCR?

I was wondering which are the SYBR green miR controls that are usually used? I'm using RNU6-B but I don't get the same Ct values on my samples, even if they are cultured samples. I'm using...

27 May 2016 5,180 2 View

Does someone work with endometrial cell lines, e.g. HESC or St-1?

I work with both lines, but it is very difficult to expand them under standard conditions.

19 March 2015 3,529 3 View