I need to extract RNA from hybridoma cells and I was wondering if the viability of the cells matters for this purpose. I have not found any paper that mentions this when describing their RNA extraction procedure.
RNA is degraded relatively quickly when cells die (either via apoptosis or just lysis), so I would say yes: viability matters. You don't get much RNA from dead cells.
Plus unhealthy unhappy cells, even if still viable, will present a different transcriptome to that of happy healthy cells.
For example, serum starved hybridomas would exhibit stress responses not found in cells grown in rich media. And so on.
RNA is degraded relatively quickly when cells die (either via apoptosis or just lysis), so I would say yes: viability matters. You don't get much RNA from dead cells.
Plus unhealthy unhappy cells, even if still viable, will present a different transcriptome to that of happy healthy cells.
For example, serum starved hybridomas would exhibit stress responses not found in cells grown in rich media. And so on.
you can get the better RNA yield if you had hybridomas in a healthy condition. Go for the RNA extraction from hybridomas which are in a log phase and also after 2-3 passages.
all the best
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