I have recently done some inhibition experiments with MRTX849 and AMG510 in MiaPaCa-2, H358, HCC44 while looking at the phosphorylation of ERK1/2 and S6.

Once over 24h and once over 96h with concentrations of 1nM, 10nM, 100nM and 1000nM and a DMSO control.

Strangely, the bands of my western blot do not match the concentration of the inhibitors. Now I wonder what could be the reason for this?

Maybe someone has an idea? Thank you very much!