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Questions related from Fabian Recker
Good evening, I am wondering why sometimes I only have very few cells on my cover slip, after staining. Could it have something to do with my washing procedure? I allways wash 3x with 1ml pbs in...
01 July 2022 5,431 3 View
Good evening, so my question is about chemilumiscence. So when I use a rabbit primary antibody for.my phosphorylated protein, put the rb-HRP 2. antibody on it and envelope it with ECL-solution,...
17 May 2022 7,489 1 View
I have recently done some inhibition experiments with MRTX849 and AMG510 in MiaPaCa-2, H358, HCC44 while looking at the phosphorylation of ERK1/2 and S6. Once over 24h and once over 96h with...
23 January 2022 554 0 View
Good Evening, I have a question about the normalisation of my results when measuring phoshorylated proteins. Example: EGF stimulation experiment: I measure phoshorylation of AKT as a function...
31 December 2021 3,901 1 View
I have a problem with loading my sds gel with the Hamilton syringe: Air bubbles appear when I load my sample with the syringe. When applying the sample I have the feeling that a lot of sample...
06 December 2021 8,612 0 View
Good Afternoon, the Blot was blocked in 3%BSA/TBST for 1hour, then treated with Akt1/2/3 (SantaCruz, 1:500 in 3%BSA/TBST), ms HRP and ECL, and then developed in Fusion FX. Due to the high...
30 November 2021 3,340 3 View
Maybe you have some ideas about where this backround is coming from? I am not sure if this comes from blotting, blocking or if this is dirt? Thank you very much in advance!
09 November 2021 8,643 3 View
