Fabian Recker

5 Questions 1 Answers 0 Followers

Questions related from Fabian Recker

Immunofluorescence: Very few cells on the coverslip?

Good evening, I am wondering why sometimes I only have very few cells on my cover slip, after staining. Could it have something to do with my washing procedure? I allways wash 3x with 1ml pbs in...

01 July 2022 5,412 3 View

How to show pshopho-proteins/unphosphorylated-proteins in Chemilumiscence without overlapping?

Good evening, so my question is about chemilumiscence. So when I use a rabbit primary antibody for.my phosphorylated protein, put the rb-HRP 2. antibody on it and envelope it with ECL-solution,...

17 May 2022 7,463 1 View

Normalization of phoshorylated proteins in Western Blots?

Good Evening, I have a question about the normalisation of my results when measuring phoshorylated proteins. Example: EGF stimulation experiment: I measure phoshorylation of AKT as a function...

31 December 2021 3,875 1 View

High backround in westernblot with chemoluninescence?

Good Afternoon, the Blot was blocked in 3%BSA/TBST for 1hour, then treated with Akt1/2/3 (SantaCruz, 1:500 in 3%BSA/TBST), ms HRP and ECL, and then developed in Fusion FX. Due to the high...

30 November 2021 3,322 3 View

"Dirty" and speckled backround on my western blot?

Maybe you have some ideas about where this backround is coming from? I am not sure if this comes from blotting, blocking or if this is dirt? Thank you very much in advance!

09 November 2021 8,629 3 View