Other than HSV-1 and Dengue virus, are there any other viruses known to interfere with mitochondrial DNA?

Reports have shown that HSV-1 UL12.5 protein degrades host mt DNA and Dengue virus NS2B targets cGAS for degradation to block IFN production caused by cellular damage and mt DNA leakages. I am...

07 August 2017 7,494 2 View

Analysis of intracellular YFP protein in primary cells by flow cytometry and immunohistochemistry of cryosections?

02 March 2016 7,142 6 View

Any evidence that IAV infection induces acute lung injury which might be the main cause of death in mice?

I would appreciate if anyone could suggest me a published article showing that IAV infection in mice causes lung injury which results in death. Much appreciated

08 September 2015 1,246 2 View

Trying to perform cloning using CRISPR/Cas9 kit but it seems as if the efficiency is very low with the kit or system. Has anyone experienced this?

CRISPR/Cas9 system is a system normally used by some microorganisms to repair DNA defects in their genome. This system is now used as an efficient tool for genome engineering. I am currently...

03 April 2014 7,539 6 View

What is the difference between blunt-ended dsRNA and dsRNA with overhangs (viral RNA)?

It might just be a question of semantics but I would appreciate if somebody could help clarify this. Is the difference (only) the fact that overhang dsRNAs have unpaired nucleotides at the end and...

31 December 2013 7,345 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View