Dacquin Kasumba

6 Questions 17 Answers 0 Followers

Questions related from Dacquin Kasumba

Other than HSV-1 and Dengue virus, are there any other viruses known to interfere with mitochondrial DNA?

Reports have shown that HSV-1 UL12.5 protein degrades host mt DNA and Dengue virus NS2B targets cGAS for degradation to block IFN production caused by cellular damage and mt DNA leakages. I am...

08 August 2017 7,536 2 View

Analysis of intracellular YFP protein in primary cells by flow cytometry and immunohistochemistry of cryosections?

I am trying to detect EYFP signals from Ifnbmob mice (made by Richard M. Locksley, Univ of California San Francisco-HHMI) tissues. These mice co-express Ifnb1 and EYFP from the Ifnb1 locus. The...

03 March 2016 7,192 6 View

Any evidence that IAV infection induces acute lung injury which might be the main cause of death in mice?

I would appreciate if anyone could suggest me a published article showing that IAV infection in mice causes lung injury which results in death. Much appreciated

09 September 2015 1,289 2 View

Does someone have evidence to support that polyIC could induce antiviral innate immunity activation in vivo (animal models) through RLRs or TLRs?

PolyIC has been used extensively in in vitro experiments to mimic viral infection in cell lines. Can anyone please refer me to an article or publication/document showing that polyIC can be used in...

09 September 2014 587 4 View

Trying to perform cloning using CRISPR/Cas9 kit but it seems as if the efficiency is very low with the kit or system. Has anyone experienced this?

CRISPR/Cas9 system is a system normally used by some microorganisms to repair DNA defects in their genome. This system is now used as an efficient tool for genome engineering. I am currently...

04 April 2014 7,578 6 View

What is the difference between blunt-ended dsRNA and dsRNA with overhangs (viral RNA)?

It might just be a question of semantics but I would appreciate if somebody could help clarify this. Is the difference (only) the fact that overhang dsRNAs have unpaired nucleotides at the end and...

01 January 2014 7,416 2 View