What is the difference between blunt-ended dsRNA and dsRNA with overhangs (viral RNA)?

More Dacquin Kasumba's questions See All

Other than HSV-1 and Dengue virus, are there any other viruses known to interfere with mitochondrial DNA?

Reports have shown that HSV-1 UL12.5 protein degrades host mt DNA and Dengue virus NS2B targets cGAS for degradation to block IFN production caused by cellular damage and mt DNA leakages. I am...

07 August 2017 7,557 2 View

Analysis of intracellular YFP protein in primary cells by flow cytometry and immunohistochemistry of cryosections?

I am trying to detect EYFP signals from Ifnbmob mice (made by Richard M. Locksley, Univ of California San Francisco-HHMI) tissues. These mice co-express Ifnb1 and EYFP from the Ifnb1 locus. The...

02 March 2016 7,216 6 View

Any evidence that IAV infection induces acute lung injury which might be the main cause of death in mice?

I would appreciate if anyone could suggest me a published article showing that IAV infection in mice causes lung injury which results in death. Much appreciated

08 September 2015 1,307 2 View

Does someone have evidence to support that polyIC could induce antiviral innate immunity activation in vivo (animal models) through RLRs or TLRs?

PolyIC has been used extensively in in vitro experiments to mimic viral infection in cell lines. Can anyone please refer me to an article or publication/document showing that polyIC can be used in...

08 September 2014 606 4 View

Trying to perform cloning using CRISPR/Cas9 kit but it seems as if the efficiency is very low with the kit or system. Has anyone experienced this?

CRISPR/Cas9 system is a system normally used by some microorganisms to repair DNA defects in their genome. This system is now used as an efficient tool for genome engineering. I am currently...

03 April 2014 7,597 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View