Dear fellow scientist,
For RNA isolation I use Trizol, however is pH of Trizol between 4- 6 which allow
DNA to be retained in the organic phase and interface, leaving the RNA in the
aqueous phase?
Greets,
Tse
Acid phenol specifically leaves RNA in the aqueous phase. Low pH is crucial since at neutral pH DNA not RNA partitions into the aqueous phase. Check the pH of old TRIZOL/TRI reagents!
low pH is better for RNA , so u can use 4.7- 5 pH of your reagent .
Dear all,
Thank you all I very appreciate your responses. I tried it with a pH indicator paper, but unfortunately it dissolve the color of indicator paper. Another colleague said that Trizol is already optimal for RNA isolation and assume pH is in the good range which additional acid phenol was not necessary.
Greets,
T
For those who are asking similar questions:
According to the analytical specifications of Trizol, 1/10 dilution (in water, at room temperature, I suppose) should have pH of 4.8-4.9 i.e. pH 3.8-3.9
I tried this as a quick check to see if our really old trizol is still usable.
Reference:
https://www.interchim.fr/ft/D/DU1295.pdf#page=19&zoom=100,77,174
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