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Questions related from Sandeep Kumar Swain
Hi Everyone, Hope you are doing well, currently i am working on ARMSPCR study to detect some mutation, but I am unable to understand how can I take a positive control for my study if earlier i...
15 July 2024 2,517 4 View
generally we use to do nutritional parameter study by the crude material of plant, but I want to know if we will take any organic solvent extract of any plant material can we do the nutritional...
30 May 2017 7,119 4 View
I did some plant hydroalcohol extract , now i want run the TLC , even i have tried more than 20 solvent system for isolation of some flavonoid , i am unable to get anything yet. so please i need...
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i am doing Assay of sucrase inhibitory activity (in-vitro), already tried a lot with different methods , i am using the Sucrase enzyme from Sigma. but i am not getting the result. so somebody can...
17 January 2017 2,304 1 View
i am studying antioxidant activities of some plants , i am doing different assay but this Hydroxyl radical scavenging is just not coming properly. i am unable to trouble shoot , so need some...
18 November 2016 8,984 7 View
My sample extraction is done by Ethyl acetate , now i am facing problem while doing TLC profiling so how can i remove chlorophyll.
14 September 2016 4,812 8 View
Hi, i am doing some study on Inhibition assay for α-glucosidase activity of a plant extract , here the reagents i am using α-glucosidase( 0.02 unit, i.e 5 micro liter) PNPG 5mm(50 micro...
01 January 1970 3,976 4 View
Hi, Greetings to all of you. i am trying to do a western blot analysis of some of my target protein but each time i am ending up with so many non specific binding results. don't know how to get...
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Except STZ and High fat diet induction model, what esle best model is availbale for induction of Type II diabetes in Rat.
01 January 1970 8,903 5 View