Does somebody know sample variations of extracted DNA amount from DBS?

25 January 2017 1 3K Report

I am looking for previous reports that showed sample variations of extracted DNA amount (and quality) from DBS card. There might be huge variations of DNA amount as well as quality of the DNA. This would be very important to know before we amplify target genes from the samples.

Bishwanath Chatterjee

I don't have direct experience with this but this slide presentation could be helpful.

Thanks

https://www.aphl.org/programs/newborn_screening/Documents/2015_Molecular-Workshop/DBS-DNA-Extraction-Validation-and-Quantification.pdf

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