Does anyone know if sodium periodate interacts with DSPE-PEG? I am seeing an interesting trend of the fluoresence of flurorescently labeled DSPE peg going down in the presence of NaIO4.
Any insight would help
I'm trying to extract RNA from Avian vaccines such Newcastle and Infectious Bronchitis, the brand of the vaccines is (VALVOC), so far I didn't extract any RNA,
27 November 2023 6,493 0 View
Hi y'all, I am running into some difficulties with qPCR (SYBR). To give y'all a brief background summary, I transfected some cells (ASO) and am running qPCR to see its effect on known genes using...
08 November 2023 9,775 6 View
total Dna extraction from poultry tissue, kidney, liver, heart using spin column.
25 September 2023 493 6 View
Hello I found in some publication that the gain and the efficiency of microstrip antenna could be ameliorated by making a slots in the ground. why the slots improve the gain and the efficiency...
15 January 2023 2,946 0 View
I want to prepare a Rose bengal test
16 December 2022 255 0 View
Hello, When I connect my antenna (2.45 GHz with output power of -10 dBm) to the rectifier (2.45 GHz) there is no output voltage, however when i injected the same power (-10 dBm) in the same...
26 October 2022 9,109 11 View
Hello, What is the difference between the simulation on ADS schematics and layout simulation for S parameters? In layout simulation the Momentum is used to get the S parameters, in schematics how...
19 September 2022 5,876 2 View
As titled, the diode in my half-wave rectifier did not rectify the AC signal. Vout is still AC signal. i am using diode SMS 7630.
21 July 2022 1,941 5 View
I followed the Thermo Scientific steps for preparing the ellmans reagent solution. When added 4g of Ellmans reagent to the 1000 uL buffer solution, the solution turned a faint pale yellow. Is this...
22 March 2021 4,602 3 View
What is the most clear and straight forward way of doing qualitative analysis for EFL students conversation transcriptions?
20 August 2019 3,459 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
Hello everyone, I am running DFT with CP2K software at 1200 K, 1 bar with a periodic system of 64 Al atoms + 2 CO2 atoms. However, I am seeing my temperature slowly increase to >4000 K and my...
22 July 2024 3,177 0 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
I want to develop the figure like attached figure. How can I get, peak period and significant wave height at any given location.
13 July 2024 886 2 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View