total Dna extraction from poultry tissue, kidney, liver, heart using spin column.
Talk to another lab member. With no labels, all I can say is "some of those samples have more nucleotides than others".
If you really want to know the concentration, use a nano drop or Qbit.
Good luck!
It depends on how much sample you loaded and it also depends on how you standardized it for each sample. Or at the final stage do you normalize everything using any calculations.
All the best.
Sid Ahmed Kendouci
Upper gel:
In samples 3 & 4 there is obviously no or very little DNA.
In my opinion all other DNAs are not properly dissolved and the gel is overloaded; and you might have RNA contamination.
Ensure your DNAs are completely dissolved and as suggested by others determine the concentration and reload equal amounts.
And, as also suggested by others, you should always seek advice from your supervisor or a senior lab member.
Well, it's a bit of an unknown outcome, it would help to label your lanes so we can determine if there is a pattern for which sample types give which gel outcomes. I agree with talking to another lab member, and you certainly have some kind of degradation and/or RNA carryover. What are you hoping to do with your samples?
The quality of your DNA looks good, but before you get to gel electrophoresis, it's better to quantify using spectroscopy.
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