I have a cell experiment and need to use a sterile round bottom W384-well plate, but most of them are labeled as non-pyrogenic instead of sterile. Does non-pyrogenic mean sterile? If not, what is the specific difference between them?
Hello Qiangyun Ai
No, non-pyrogenic does not mean sterile. Sterility and non-pyrogenic are different from each other. This is because sterility just ensures that there is no live microorganism. The sterilization process makes a product sterile. In other words, it is a process of killing or removing bacteria and other forms of living microorganisms such as fungi and viruses and their spores.
On the other hand, pyrogens are not bacteria, but they are bacterial cell wall fragments. Typically, if bacteria mainly referring to gram-negative bacteria are destroyed during the sterilization process, endotoxin will be released and will remain undetected unless endotoxin testing is done. Endotoxins cannot be accurately detected through sterility testing. Being chemically stable, pyrogens are not necessarily destroyed by conditions that kill bacteria. For this purpose, depyrogenation is necessary to make a product pyrogen-free.
So, you may have products that may be sterile or sterile and pyrogen-free or pyrogen-free but non-sterile. So, the round bottom W384-well plate labeled as non-pyrogenic instead of sterile may mean pyrogen-free but non-sterile.
Best.
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