What can be the probable buffer composition to transfect in Jurkat cell line using electroporation?

I am trying to transfect my protein in Jurkat cell line but following a lot of issues. I am using 1X PBS as transfection agent, but the transfection level is low. Can anyone kindly suggest some...

29 July 2020 8,176 1 View

Problem is in running Gaussian 09 job (Windows). After submitted the job is immediately deleted. The check-route says 'file is not specified'. Why ?

I am using Gaussian 09 (windows). The problem has generated over last few days. Whenever I submit the job, it is immediately deleted with no error message and the output file is empty. The...

10 July 2020 2,604 1 View

How would I make hypercholesterolemia in Mice?

I am working on Mice and want to make hypercholesterolemia. But after giving cholesterol powder the level of cholesterol has decreased from previous level. Could anyone please suggest me how will...

26 February 2020 9,691 3 View

Why hasn't geopolymer based concrete been used that much?

OPC is the 2nd largest generator of CO2 after automobile industries. Researchers have claimed that geopolymer based concrete is more stronger , durable,versatile and eco friendly but...

04 March 2019 8,312 20 View

Which of the Clathrin Anitbody works well with murine cells?

Hello everyone, I have been using D3C6 from cell signaling and MA1-065 (monoclonal X22 from Thermofisher. I feel that they donot work well.Can you please tell me about other antibodies besides...

08 January 2017 3,616 4 View

Could Any one suggest for chromium estimation by UV Vis method using DPC?

Cr(vi) using DPC I mean what should be the ppm level to get a calibration curve. I tried but more than 10ppm the complex is very weak and gradually disappears. Any comment/suggestion is appreciated.

24 July 2016 1,559 3 View

Is the chemical synthesis of peptides necessary?

I am working on generating bioactive peptides from a natural source. I have read a few articles where people chemically synthesize the peptides after they have identified it in their bioactive...

10 March 2015 3,720 8 View

Can anyone recommend a pressure cell for transmission spectroscopy on polymer thin film under pressure?

I am in search of a pressure cell with optical (mostly visible wavelength regime) windows, where I can place a soft polymer thin film and squish it under controlled calibrated pressure for...

04 December 2012 7,150 9 View

What is the impact of climate change on indigenous people and isolated/ marginal communities in Mountains?

Climate change has impacted everywhere, every-sector and peoples.The most vulnerable group are mountain communities. Therefore it would be good to know about the case studies if available.

27 August 2012 7,281 3 View

Does it make sense to think of a completely empirical Physics dispensing altogether with the presiding Metaphysics ?

01 January 1970 7,367 60 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

What condition required for coating of Fe3O4@SiO2 by using APTES?

I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks

24 February 2021 5,992 4 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View