Dear all,

we have tried for several times evaluation of motility (by CASA) and other functional parameters of sperm recovered from drops of media under mineral oil (Sigma, embryo tested). However we got really different results of motility and e.g. viability and acrosomal integrity. Sperm motility were approx. 30% lower than viability. Moreover there was huge difference also in comparison with samples recovered from microtube.

We found effect of chamber thus we are using only one type, with the lowest effect on motility.

We also remove rest of oil from tip before loading to chamber.

Does anybody have suggestion?

Thank you.

Ondrej