18 August 2015 2 4K Report

Microglia and NG2-glia survived the same treatment while astrocytes do not seem to withstand it! Anybody else made a similar observation?

More Hari Sapkota's questions See All
In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Similar questions and discussions
Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Can zinc based fixatives be used after enzymatic dissociation of neural tissue?

I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can...

21 January 2021 4,430 1 View

Immunostaining of common bacteria in FFPE samples?

Dear Collegues, If we want to perform immunohistochemistry /immunofluorence staining from FFPE human biopsy (vein) samples and study the presence of LPS and or common pathogen like Porphyromonas...

01 December 2020 5,744 2 View

Experience measuring human LPS in plasma?

Hello, Has anyone had luck measuring LPS from human plasma? Which ELISa kit do you recommend? I am getting a lot of interference from color of the plasma. Thanks

15 November 2020 6,599 1 View

How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

What's the best way to detect pro-inflammatory cytokines in RAW264.7 supernatant?

Hello! I'm starting my Master's project soon, and I'm in the optimization/practice step. I'm stimulating RAW264.7 cells (in a 24 well) with LPS for 18h. I did a first experiment today: a...

21 October 2020 2,761 8 View

Can someone recommend a flow cytometry protocol to sort astrocytes and microglia?

I would like to use flow cytometry sorting (Aria III) to sort astrocytes and microglia from adult human and adult mouse brain, with the aim of extracting RNA from sorted bulk pools. Can someone...

11 October 2020 3,624 1 View

Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,517 1 View

Do you have any suggestion for a easy and cheap experiment to quantify Cytokines?

I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...

27 September 2020 5,366 6 View

First time project advice: How to close transcription factors, SOX9 and NFIB, into baculoviral vector, pEZT-BM?

Hi all, I am new here and I am about six weeks in to my first ever research project! (I just came here to kick-start my career as a researcher.) :) The aim of my research project: "The aim...

15 September 2020 9,890 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close