Hari Sapkota

4 Questions 2 Answers 0 Followers

Questions related from Hari Sapkota

How do you prepare a high osmolarity 4% PFA for fixing 300 um brain slices?

I am trying to use 300 um hippocampal sections for immunohistochemistry but the antibodies have not penetrated deep enough (

26 October 2021 1,284 0 View

How do you distinguish stimulus artifact vs fibre volley vs fEPSP signal in a field potential recording?

I am taking field potential recordings from stratum radiatum in the CA1 region by stimulating Schaffer collaterals. Sometimes I can't quite tell where the artifact ends and fEPSP signal begins,...

10 August 2019 4,661 2 View

Does LPS treatment (10 ug/mL) for three days kill astrocytes?

Microglia and NG2-glia survived the same treatment while astrocytes do not seem to withstand it! Anybody else made a similar observation?

18 August 2015 4,407 2 View

Does CD68 specifically mark activated microglia?

LPS (1ug/mL)-stimulated microglia are positive for CD68, but so are Vehicle (serum-free medium) treated microglia. I used fluorescent microscopy to visualise them. Fluorescence intensity for CD68...

13 August 2015 8,432 7 View