How to perform right tailed Mann Whitney U test in spss?

I want to check H0 : Both male and female students equally likely believe that programming is an interesting subject H1 : male students believe more than female students that programming is an...

05 August 2020 5,236 3 View

How to decide the side of one tailed Mann Whitney U test?

I'm doing a research "perception towards programming based on gender." I've written the null hypothesis H0 : Both male and female students equally likely believe that programming course is...

27 July 2020 2,534 3 View

Charcoal carbon paste electrode

Has anyone made a carbon paste electrode using activated charcoal? What is the advantage of using graphite over activated charcoal to construct a CPE? Also Is there a way to remove the impurities...

18 June 2017 9,245 2 View

What are essential preprocessing steps involved in getting historical aerial photographs usable?

23 May 2015 8,003 3 View

Anyone have recommendations for the preliminary planning of site ground truthing?

I am working on land degradation detection. My research site is an arid, mountainous region. Grazing is the dominant human factor intervening with natural vegetation. The sites selected are less...

22 February 2014 3,621 3 View

Where can I find a bank cheque image database for my research?

I'm hoping to do research on automated bank cheque verification system for my final year project using machine learning algorithms (deep learning).

19 February 2014 4,967 3 View

Can perform the basophil activation test using healthy individuals' basophils?

I am looking for an alternative way of doing basophil activation test(BAT) using passive sensitization. Technically, sensitizing the besophils from the healthy individuals' by patients sera and...

01 January 1970 1,416 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View