Is there any way to design allele specific PCR primers for a single nucleotide "deletion"?

Hi, I am currently doing a research on Inborn errors of metabolism in Sri Lanka and for that, I need to develop a method to identify few SNPs and a single point deletion using allele specific PCR...

27 August 2020 2,411 4 View

How to perform right tailed Mann Whitney U test in spss?

I want to check H0 : Both male and female students equally likely believe that programming is an interesting subject H1 : male students believe more than female students that programming is an...

05 August 2020 5,236 3 View

How to decide the side of one tailed Mann Whitney U test?

I'm doing a research "perception towards programming based on gender." I've written the null hypothesis H0 : Both male and female students equally likely believe that programming course is...

27 July 2020 2,534 3 View

How to evaluate the accuracy of unupervised machine learning algorithms ?

What are the ways that we can use to analyze the accuracy of unsupervised anomaly detection algorithms like HBOS or Isolation Forest ?

24 February 2020 5,393 3 View

How to Encode an IBAN Number ?

My dataset has an IBAN number column with the following format. IBAN Number Example :- "GB38MIDL400530543464" What is the best way to encode the IBAN number column before training the dataset?

02 February 2020 2,793 3 View

Do any one knows about Non linear modeling techniques for behavioral modelling?

I'm exploring non linear modelling technique for demand forecasting in transportation sector where alternative transport mode implement. As a start it is good to have some ideas from you Thank you

18 October 2018 7,019 1 View

Suggestions for the questionnaire ?

Currently I am doing a research on Customer Retention Hotel Service Attributes in Sri Lanka: Effect of Customer's Nationality - Case of Cinnamon Grand Hotel. with the support of literature i...

11 December 2017 5,823 7 View

Does KU 812 cell line show a self differentiation to mature basophils?

24 September 2017 1,706 2 View

Charcoal carbon paste electrode

Has anyone made a carbon paste electrode using activated charcoal? What is the advantage of using graphite over activated charcoal to construct a CPE? Also Is there a way to remove the impurities...

18 June 2017 9,245 2 View

What are essential preprocessing steps involved in getting historical aerial photographs usable?

23 May 2015 8,003 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View