Do we need to incubate suspension cell before treatment with drugs (or any stimulants)? As I know for adherent cell, it is required so because it need time to get attached. Does it make any difference if we treat directly without pre-incubation?
You can treat your cells directly. I don't see the reason to make preincubation.
Cells either attached or in suspension are usually pre-incubated with serum restricted (low) or serum free isosmotic buffered solutions to establish the cultures into a synchronized phase with less or little cytokines, that could possibly interfere with the drug action or specifically if the target is cytokines or other immunological molecules.
If you are sure that the drug or treatment conditions you are planning to employ do not interfere with the growth media containing serum present in the suspension culture media you can certainly go for direct drug treatment.
Hope that answers your question!
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