I have read that some article distinguished between proliferation and migration in Wound Healing Test.
But does HaCat (keratinocyte cells) need the differentiation as it does not contain any muscle cells, only endothelial / keratinoctye cells.
Example of Migration Analysis done using treatment of pure compound (rutin, hesperidin, quercetin, corylin, morin and allantoin) in concentration of ug/ml. Would the unit ug/ml affected the...
11 July 2023 5,968 1 View
Working on understanding the mechanism of neurotoxins and making its dataset for toxicophore analysis
07 February 2023 9,946 2 View
I have tested paracetamol using UV-Vis characterization and the result of paracetamol maximum wavelength is 243 nm, the result is same with the literature. But, why does paracetamol have a maximum...
16 October 2022 9,173 6 View
Dear Everyone, 1. How to convert GDS , BDI 11, ADI -2 , PHQ-9 raw -score to Z score ? so that i can have standardized score to calculate the mood disorder in my study ? - Could you share links...
16 October 2022 3,889 2 View
Hi everyone, i have work using electrochemical method. I tested before and after treatment my sample using FTIR and the spectra shown in the "FTIR-2" image, with the blue curve is untreated and...
21 February 2022 4,811 6 View
Hi everyone, i have work using paracetamol sample with electrochemical wastewater treatment under special conditions, then analyze with COD (Chemical Oxygen Demand) removal, UV and FTIR. Analyze...
30 January 2022 2,804 4 View
I am new to Organizational Network Analysis and my lecturer told me to seek for a software that does ONA, whether the software is free of charge or not it does not matter. Thank you in advance.
28 December 2021 9,090 10 View
Assalamualaikum and hi, I need help on how to calculate sample size (for each group; treatment and control groups) for quasi experimental, using repeated measures MANOVA? Thank you so much for...
18 June 2021 5,102 4 View
I am writing a research proposal and I have to analyze my data. My research is about student's time management and its relation to academic performance. I am using Britton and Tesser, Time...
26 December 2020 7,014 3 View
For Artificial Neural Network, I already tried many structures and the best result was used the number of hidden layer neurons same with the input layer neurons. Is there any special reason for that?
27 January 2020 4,907 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View