I have read that some article distinguished between proliferation and migration in Wound Healing Test.
But does HaCat (keratinocyte cells) need the differentiation as it does not contain any muscle cells, only endothelial / keratinoctye cells.
I am writing a research proposal and I have to analyze my data. My research is about student's time management and its relation to academic performance. I am using Britton and Tesser, Time...
26 December 2020 6,883 3 View
I'm using Gamry instrument interface 1000 and it obviously only state current I've tried to calculate the current density (A/g) by simply current divided by the mass of electrode (without...
09 November 2019 6,809 1 View
I am interested in education research, sometimes i found that using of t-test is common in educational research. In my finding almost undergraduate student will chose t-test to analyze their...
31 May 2018 2,927 5 View
Hi, I am studying on the effect of activated carbon particle size to supercapacitor electrode physicochemical and electrochemical properties. I am planning to use laser diffraction particle size...
17 July 2017 6,443 3 View
I'm doing my research about supercapacitor using neutral aqueous electrolyte. Recently, I've found there are some researches use activated carbon (e.g: Kuraray) as counter electrode in neutral...
14 March 2017 448 3 View
please answer this based on the pharmacological point of view, thank you.
21 October 2016 918 3 View
What is the difference between shock and strut? Do wheel have to be realigned when strut are replaced?
27 February 2016 8,679 1 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
28 February 2021 2,534 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View