How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View

How to report data about studies awaiting classification in a Systematic Review?

Hi All, I am currently undertaking a systematic review. 19 studies were classified as studies awaiting classification. I have contacted the authors. Several authors did not reply and a number...

22 July 2019 3,987 1 View

How should I compare sEMG data across different clinical groups?

Hi All, I am new to the field of sEMG so any guidance or feedback would be greatly appreciated. I am collecting facial EMG data from two groups: healthy vs individuals with neurological impaired...

19 June 2019 6,067 3 View

How should similar studies by the same authors be assessed in a systematic review?

Hi All, I am currently undertaking a systematic review. Two of the studies, completed by nearly the same authors, seem to have used the same set of participants. The differences mainly lies in...

30 May 2019 7,017 8 View

Is there any robust assay to quantify the maturation state of autophagosomes in vertebrates?

I am looking for a way to quantify autophagosome maturation before fusion with lysosomes to see if there is any defect in cargo recognition. I've read about co-localization studies of p62 and LC3...

26 May 2019 2,200 1 View

AMBER parameters for Boron (in BDPY-linked-ligand)?

Boron atom type is not present neither in GAFF neither in AMBER force field. Is there any ff implementation to parameter Boron? Do you have any suggestions to solve out the problem?

15 January 2019 7,264 10 View

How can I optimize a PCR of a 5 kb fragment using Q5 polymerase?

I have amplified this 5 kb fragment using LongAmp Taq DNA polymerase from genomic DNA, then I extracted the amplified product from the agarose gel and I have purified it. I have tried amplifying...

20 April 2017 1,380 2 View

What statistical test to use?

16 December 2015 8,657 12 View

How do I control genetic maps quality?

I have a map of a plant BC1 population i have drawn with MAPDISTO software and re-drawn with joinmap4.1 software. Both maps show linkage between two arabidopsis chromosomes probably due to...

01 October 2015 4,470 5 View

I have a soil sample and I want to distinguish how much of bacteria, fungi and plants are present. Any suggestions?

I need a precise detection technique to detect bacteria, fungi, plants in soil.

22 September 2013 6,979 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View