Pasquale Primo

2 Questions 1 Answers 0 Followers

Questions related from Pasquale Primo

How can I optimize a PCR of a 5 kb fragment using Q5 polymerase?

I have amplified this 5 kb fragment using LongAmp Taq DNA polymerase from genomic DNA, then I extracted the amplified product from the agarose gel and I have purified it. I have tried amplifying...

21 April 2017 1,448 2 View

Does Gibson Master Mix include vector?

i 've read the protocol about Gibson Master Mix but I can't understand if the plasmid is already in the Gibson Master Mix.

24 June 2016 3,652 1 View