I have to send my sample for metagenomics sequencing but my genomic DNA shows up a clear band with thin and long smear. It looks like my sample has degraded but I'm not sure. I read somewhere that it's quite common for genomic DNA to have smear. But I fear the sequencing lab would reject my sample.
So far, this is the best result I can get and I'm tight on schedule so it's almost impossible for me to optimized another extraction method.
Do you think my sample are qualified for the sequencing? My sample is lane 1 and lane 2 (I mistakenly cropped off the ladder, though).
I need your advices. Thankyou so much!
Kathleen Nad Hello as per shown picture your DNA seams to fragmented and it might be possible during isolation process & its risky to further process with Sequencing.
Hope it might help u..
Hi Kathleen, in the first two lanes, the band is of relatively high intensity. Though there is smear which is indicating fragmentation. I still think you can proceed to library preparation with these samples. I am assuming you are going to do short-read Illumina sequencing only so slight fragmentation would not affect your sequencing run. Also, once the library is prepared, you can do a QC of it using a bio-analyzer or tape-station before loading it on the sequencer.
Thankyou Nagesh and Abhinav, unfortunately my sample is rejected for further analysis. I was told the bands were too thin and would be risky to be sequenced.
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