Does gene containing intron enhances the Heterologous gene expression in filamentous fungi?

More Pankajkumar Ramdas Waghmare's questions See All

Which factors studied for Sayajirao Gaekwad II: Economic Thoughts and Transformation as the Maharaja of Baroda ?

Explain some Economical indicators for Sayajirao Gaekwad II: Economic Thoughts

29 February 2024 9,256 0 View

What are the techniques for mycotoxin level and detoxifying enzymes detection in infected leaf sample ?

how we can do the quantitative detection of mycotoxin produced by Alternaria brassicae

04 February 2020 7,122 3 View

NF Kb estimation: any available biomolecule in serum which can estimate the expression of NF-KB?

nuclear factor kappa light chain enhancer of activated B cells which gives estimate of cytokine release, ultimately inflammation . Is there any diagnostic kit is available which can estimate its...

28 July 2019 2,036 5 View

Which inflammatory marker is better, CRP or Procalcitonin?

Newly emerged Procalcitonin is gaining attention as a potential marker of inflammation. Does it is really good marker for all type of systemic inflammation particularly for impending...

06 February 2019 9,572 7 View

Do we have Risk assessment tool at patient's home?

Recent Decades most of long term Patient care is being carried out at patient home. This gives rise to many challenges to provide quality care as per patients condition. we are caring in patient...

02 February 2019 4,274 1 View

What is difference between Neubauer chamber and Petroff Hausser chamber?

Both of these are used for cell count. If we see the dimensions , they are also same, (3mm x3mm) with other technical markings on chamber. Then what is difference? Can acid fast bacilli (AFB )...

03 January 2019 6,838 2 View

Why insulin is given in type 2 diabetic patient as it is insulin resistant diabetes?

One of mode for treating type-2 diabetes is insulin injection. As per the pathophysiology there is insulin resistance to insulin for extra-hepatic tissues resulting in hyperglycemia. Does insulin...

13 December 2018 2,104 17 View

Which parameter does control stiffness degradation in ABAQUS Concrete Damaged Plasticity Model for triaxial Loading case?

I am trying to verify the experimental results on triaxial compressive loading on concrete cylinder using Concrete Damaged Plasticity Model, but I am not able to get results as expected.. Getting...

16 September 2018 2,909 5 View

Is pAc5.1 plasmid low copy number plasmid?

I consistently get low yields with pAc5.1 constructs. I have tried growing cultures from fresh plates and glycerol stocks. I have also tried to use starter cultures (grown for 8 h) and I always...

05 June 2018 8,337 3 View

What is relative velocity of structures during earthquake excitation?

what is relative velocity of structures during earthquake excitation? if we know the horizontal acceleration and max horizontal placement.

26 October 2017 6,217 1 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

What is gangrine? Is there gangrine happened indie potato?

Please discuss someone told about fungus and allied others.

31 July 2024 3,372 3 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

12 July 2024 7,027 0 View

I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

Why can't we get on top of this incubator infection (fungal)?

We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...

02 July 2024 6,375 2 View

What could be the possible contaminating organism?

I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...

15 June 2024 5,515 5 View

¿Algún documento de consulta que presente claves dicotomicas para identificar especies de hongos microscopicos?

Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...

15 June 2024 6,693 0 View

Khaled Khleifat

Following

Stephen M Marek

Just off the top of my head, inclusion of an intron improved transgene expression in Armillaria ( https://www.nature.com/articles/srep29226)

See also the introduction review in Article Improved vectors for Agrobacterium mediated genetic manipula...

Saba Abdulameer kadhim Al-Ziadi

following

Navnit Kumar Ramamoorthy

Dear pankajkumar?

Are you trying the expression of a filamentous fungus's gene (inclusive of the ìnton) in another host or are you attempting expression of a gene in a filamentous fungi ?