I would like to ask whether Ganoderma boninense produces any spores when it is cultured on PDA plates?
Hi, I would like to know what is the most efficient way to observe the spores of Ganoderma ? I have tried a few method to collect the spores from Ganoderma but sometimes the microscopic view is...
08 September 2016 8,198 4 View
I would like to ask if there's anyone has encountered the chelidonic acid powder became pink in colour? Is the chelidonic acid being oxidised? Thanks.
04 May 2015 378 0 View
The Ganoderma boninense in the lab was contaminated with other fungi (which is white in color as well). I tried to subculture into different media but the contamination is still there. I would...
09 October 2014 1,722 8 View
Hi, I would like to ask for some opinion on the bioactive compounds of the extracts. Actually, is purified compound more effective in inhibiting a certain fungus/bacteria compared to crude ? Thanks.
07 August 2014 7,880 23 View
Hi, I have done the extraction from the culture filtrate of fungi using hexane. So currently I'm having the hexane extracts and I developed it using the preparative thin layer chromatography...
07 August 2014 4,385 6 View
Hi, I'm planning to observe the mycelium of fungi under SEM. But for the previous set, the fungi seems to be undergo shrinkage when observed under SEM. I wonder where went wrong. Is it due to the...
06 July 2014 8,301 13 View
I wanted to dissolve my samples with acetonitrile, but the moment I add in the acetonitrile, my sample precipitates. I don't know why it is like that. My dry extracts obtained from several...
06 July 2014 1,957 3 View
I just wanted to ask how to conduct a proper disc diffusion assay? Should I impregnate the extract to the disc first before I place it on the agar, or can I just directly pipette the extract to...
04 May 2014 4,906 4 View
I would like to ask whether it is okay if I conduct the antifungal assay by dissolving my samples in DMSO. Then for further analysis such as TLC, GCMS, I dissolve my samples in ethyl acetate? Or...
04 May 2014 5,170 1 View
I extracted my culture filtrate (cell-free) with the ethyl acetate. After I shook it for 5 minutes and let it separate, there were chunks water droplets inside the organic phase. Can anyone please...
03 April 2014 1,659 7 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View