How to observe basidiospores?

Hi, I would like to know what is the most efficient way to observe the spores of Ganoderma ? I have tried a few method to collect the spores from Ganoderma but sometimes the microscopic view is...

08 September 2016 7,911 4 View

Why the chelidonic acid powder turns slightly pink?

I would like to ask if there's anyone has encountered the chelidonic acid powder became pink in colour? Is the chelidonic acid being oxidised? Thanks.

04 May 2015 265 0 View

How can I clean up contaminated Ganoderma boninense?

The Ganoderma boninense in the lab was contaminated with other fungi (which is white in color as well). I tried to subculture into different media but the contamination is still there. I would...

09 October 2014 1,608 8 View

Does anyone have experience with purified compound or crude?

Hi, I would like to ask for some opinion on the bioactive compounds of the extracts. Actually, is purified compound more effective in inhibiting a certain fungus/bacteria compared to crude ? Thanks.

07 August 2014 7,761 23 View

How can I extract compounds from preparative thin layer chromatography?

07 August 2014 4,271 6 View

Can anyone help with why acetonitrile makes my dry extract precipitate?

06 July 2014 1,853 3 View

How can I prevent mycelium of fungi from shrinkage during the fixation for scanning electron microscopy?

06 July 2014 8,199 13 View

How can I conduct a proper disc diffusion assay?

I just wanted to ask how to conduct a proper disc diffusion assay? Should I impregnate the extract to the disc first before I place it on the agar, or can I just directly pipette the extract to...

04 May 2014 4,778 4 View

What types of solvent are used to dissolve dry extracts?

I would like to ask whether it is okay if I conduct the antifungal assay by dissolving my samples in DMSO. Then for further analysis such as TLC, GCMS, I dissolve my samples in ethyl acetate? Or...

04 May 2014 5,056 1 View

Can anyone help with extraction of metabolites in broth with solvent (ethyl acetate)?

I extracted my culture filtrate (cell-free) with the ethyl acetate. After I shook it for 5 minutes and let it separate, there were chunks water droplets inside the organic phase. Can anyone please...

03 April 2014 1,557 7 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View