18 Questions 23 Answers 0 Followers
Questions related from Lee Pei Lee Angel
Hi, I would like to know what is the most efficient way to observe the spores of Ganoderma ? I have tried a few method to collect the spores from Ganoderma but sometimes the microscopic view is...
09 September 2016 8,119 4 View
I would like to ask if there's anyone has encountered the chelidonic acid powder became pink in colour? Is the chelidonic acid being oxidised? Thanks.
05 May 2015 346 0 View
The Ganoderma boninense in the lab was contaminated with other fungi (which is white in color as well). I tried to subculture into different media but the contamination is still there. I would...
10 October 2014 1,681 8 View
Hi, I would like to ask for some opinion on the bioactive compounds of the extracts. Actually, is purified compound more effective in inhibiting a certain fungus/bacteria compared to crude ? Thanks.
08 August 2014 7,839 23 View
Hi, I have done the extraction from the culture filtrate of fungi using hexane. So currently I'm having the hexane extracts and I developed it using the preparative thin layer chromatography...
08 August 2014 4,350 6 View
Hi, I'm planning to observe the mycelium of fungi under SEM. But for the previous set, the fungi seems to be undergo shrinkage when observed under SEM. I wonder where went wrong. Is it due to the...
07 July 2014 8,267 13 View
I wanted to dissolve my samples with acetonitrile, but the moment I add in the acetonitrile, my sample precipitates. I don't know why it is like that. My dry extracts obtained from several...
07 July 2014 1,924 3 View
I just wanted to ask how to conduct a proper disc diffusion assay? Should I impregnate the extract to the disc first before I place it on the agar, or can I just directly pipette the extract to...
05 May 2014 4,854 4 View
I would like to ask whether it is okay if I conduct the antifungal assay by dissolving my samples in DMSO. Then for further analysis such as TLC, GCMS, I dissolve my samples in ethyl acetate? Or...
05 May 2014 5,130 1 View
I extracted my culture filtrate (cell-free) with the ethyl acetate. After I shook it for 5 minutes and let it separate, there were chunks water droplets inside the organic phase. Can anyone please...
04 April 2014 1,625 7 View
I would like to ask how to conduct the antifungal assay? What kind of solvent should I use to dissolve my extracts? I tried with acetone and methanol, both inhibit the pathogenic fungus (control)....
04 April 2014 925 6 View
I would like to ask whether Ganoderma boninense produces any spores when it is cultured on PDA plates?
04 April 2014 7,194 0 View
I cultured trichoderma on the PDB and extracted the PDB using EtOAc. I dried the extract using a rotary evaporator. The problem is that the extract is not dried after I rotovap for quite some...
03 March 2014 773 2 View
I would like to ask whether the MS library is able to identify the compounds in a crude extract? Or still have to go through purification before sending to the MS to identify?
03 March 2014 9,533 4 View
My dry extract does not dissolve completely in ethyl acetate or methanol also. I'm wondering if I dissolve my sample in ethyl acetate, or methanol, the results will be affected? Thank you very much.
02 February 2014 2,096 6 View
I dissolved my dry sample with ethyl acetate and ran it with TLC. It came out with very nice results; the major compound was shown on the TLC. But when I tried to dissolve my dry sample in...
02 February 2014 419 5 View
I am planning to extract the fungal metabolites which cultured from the potato dextrose broth (PDB) with solvent then run through TLC. I am just wondering, when we extracted the fungal compounds...
01 January 2014 4,015 8 View
I would like to extract my fungus metabolites and resuspend the dry extracts with methanol. After that I would like to conduct a disc diffusion assay test using the fungus methanol extract against...
01 January 2014 400 4 View
