How to observe basidiospores?

Hi, I would like to know what is the most efficient way to observe the spores of Ganoderma ? I have tried a few method to collect the spores from Ganoderma but sometimes the microscopic view is...

08 September 2016 7,911 4 View

How can I clean up contaminated Ganoderma boninense?

The Ganoderma boninense in the lab was contaminated with other fungi (which is white in color as well). I tried to subculture into different media but the contamination is still there. I would...

09 October 2014 1,608 8 View

Does anyone have experience with purified compound or crude?

Hi, I would like to ask for some opinion on the bioactive compounds of the extracts. Actually, is purified compound more effective in inhibiting a certain fungus/bacteria compared to crude ? Thanks.

07 August 2014 7,761 23 View

How can I extract compounds from preparative thin layer chromatography?

07 August 2014 4,271 6 View

Can anyone help with why acetonitrile makes my dry extract precipitate?

06 July 2014 1,853 3 View

How can I prevent mycelium of fungi from shrinkage during the fixation for scanning electron microscopy?

06 July 2014 8,199 13 View

How can I conduct a proper disc diffusion assay?

I just wanted to ask how to conduct a proper disc diffusion assay? Should I impregnate the extract to the disc first before I place it on the agar, or can I just directly pipette the extract to...

04 May 2014 4,778 4 View

What types of solvent are used to dissolve dry extracts?

I would like to ask whether it is okay if I conduct the antifungal assay by dissolving my samples in DMSO. Then for further analysis such as TLC, GCMS, I dissolve my samples in ethyl acetate? Or...

04 May 2014 5,056 1 View

Can anyone help with extraction of metabolites in broth with solvent (ethyl acetate)?

I extracted my culture filtrate (cell-free) with the ethyl acetate. After I shook it for 5 minutes and let it separate, there were chunks water droplets inside the organic phase. Can anyone please...

03 April 2014 1,557 7 View

How to conduct an antifungal assay?

I would like to ask how to conduct the antifungal assay? What kind of solvent should I use to dissolve my extracts? I tried with acetone and methanol, both inhibit the pathogenic fungus (control)....

03 April 2014 806 6 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

What is role of toluene and stearic acid in ball milling for powder ? In what weight percent we should add this to powder?

I am doing ball milling operation of Aluminum and silicon carbide powder. I am pretty confused about what should be added as process control agent ? what is role of toluene as PCA and stearic...

12 February 2021 5,205 3 View

Is there any method that we can electrochemically oxidize a powder?

I am working with a material which is sensible to water and oxygen, I would like to oxidize this powder either chemical or electromagnetically. Does anyone knows a method to electrochemically...

09 February 2021 2,944 3 View