I would like to ask if there's anyone has encountered the chelidonic acid powder became pink in colour? Is the chelidonic acid being oxidised? Thanks.
Hi, I would like to know what is the most efficient way to observe the spores of Ganoderma ? I have tried a few method to collect the spores from Ganoderma but sometimes the microscopic view is...
08 September 2016 8,198 4 View
The Ganoderma boninense in the lab was contaminated with other fungi (which is white in color as well). I tried to subculture into different media but the contamination is still there. I would...
09 October 2014 1,722 8 View
Hi, I would like to ask for some opinion on the bioactive compounds of the extracts. Actually, is purified compound more effective in inhibiting a certain fungus/bacteria compared to crude ? Thanks.
07 August 2014 7,880 23 View
Hi, I have done the extraction from the culture filtrate of fungi using hexane. So currently I'm having the hexane extracts and I developed it using the preparative thin layer chromatography...
07 August 2014 4,385 6 View
Hi, I'm planning to observe the mycelium of fungi under SEM. But for the previous set, the fungi seems to be undergo shrinkage when observed under SEM. I wonder where went wrong. Is it due to the...
06 July 2014 8,301 13 View
I wanted to dissolve my samples with acetonitrile, but the moment I add in the acetonitrile, my sample precipitates. I don't know why it is like that. My dry extracts obtained from several...
06 July 2014 1,957 3 View
I just wanted to ask how to conduct a proper disc diffusion assay? Should I impregnate the extract to the disc first before I place it on the agar, or can I just directly pipette the extract to...
04 May 2014 4,906 4 View
I would like to ask whether it is okay if I conduct the antifungal assay by dissolving my samples in DMSO. Then for further analysis such as TLC, GCMS, I dissolve my samples in ethyl acetate? Or...
04 May 2014 5,170 1 View
I extracted my culture filtrate (cell-free) with the ethyl acetate. After I shook it for 5 minutes and let it separate, there were chunks water droplets inside the organic phase. Can anyone please...
03 April 2014 1,659 7 View
I would like to ask how to conduct the antifungal assay? What kind of solvent should I use to dissolve my extracts? I tried with acetone and methanol, both inhibit the pathogenic fungus (control)....
03 April 2014 969 6 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...
14 July 2024 1,493 3 View
I am working on powder metallurgy, and during the compaction process, the powder does not pelletize and remains in its powder form. To address this issue, I am considering using binders to improve...
13 July 2024 9,003 2 View
I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?
10 July 2024 2,462 1 View
I have to compare the FTIR of the two samples, one is in powder form and other one is the powder dispersed in IPA or water. Both are same material, so how the bond will change
09 July 2024 8,011 2 View
Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...
09 July 2024 1,969 0 View
In this model clearly understanding how to active one layer to another layer and how to add powder material properties step by step.
08 July 2024 6,147 0 View
Good evening, I would like to evaluate the heavy metal content in bird feathers. Can I keep my samples in powder form ? and can I use other tubes than Teflon for digestion?
04 July 2024 158 4 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,866 2 View
I synthesise intermetallic compounds through nickel and aluminium powders. But the pure powder after synthesis is difficult to obtain. Especially the presence of aluminium oxide very seriously...
01 July 2024 4,030 3 View