Dear colleagues -
Could I be confident that adding 1% SDS (V/V) into TE buffer and boiling cultures in tubes for 10 minutes would kill any living cell?
Thank you,
Igor Brown
I think that would kill any mesophilic bacteria. There might be some hyperthermophiles that could survive it, and maybe some bacterial spores.
Yeh, I posted my question because we are dealt with thermophilic cyanobacteria. I look forward to having suggestions from the expert in this area. Igor
Yes, thermophiles have a chance of survival as mentioned by Adam B Shapiro But, if you want to denature thermophilic cyanobacteria, try using a higher concentration of SDS instead of 1%.
If you want to extract the high molecular weight genomic DNA from thermophilic cynobacterial cell you should try Gentra puregene QIAGEN kit with some modifications. I have also used for the large size genomic DNA of actinobacteria.
Pooja
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