I used HPLC to detect pure water, but there was always an unknown peak at retention time of about 11.6min. was it caused by dissolved CO2? the detector of HPLC was RID.
No. For RID detection, gas would be dissolved in the mobile phase. *Please ask your instructor for assistance with your project as the best way to answers these types of questions is to obtain some basic training, then have someone local assist you.
For Chromatography Questions: As you are new to chromatography, it helps everyone a great deal if you always include DETAILED method conditions with your initial post (e.g. flow rate, column dimensions, column brand/type/model, sample info, inj solution and volume and all detection settings and of course your detailed mobile phase composition and time table if it is a gradient method, to name a few). Without this basic information, it can be near impossible to provide constructive comments.
No. For RID detection, gas would be dissolved in the mobile phase. *Please ask your instructor for assistance with your project as the best way to answers these types of questions is to obtain some basic training, then have someone local assist you.
For Chromatography Questions: As you are new to chromatography, it helps everyone a great deal if you always include DETAILED method conditions with your initial post (e.g. flow rate, column dimensions, column brand/type/model, sample info, inj solution and volume and all detection settings and of course your detailed mobile phase composition and time table if it is a gradient method, to name a few). Without this basic information, it can be near impossible to provide constructive comments.
No any action of CO2 on RID detector.
May be some organic acid, polymer or sugar containing your water sample.
No! Sounds like contamination in your mobile phase, injector, column, detector... Remember, with RID you are limited to isocratic methods and NOT gradient.
Need more info, it likes you are askinh why my experiment doesn't work, please help. Can you help me?
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