I am recently analyzing the bile acid composition in our mice, and I observed about 2% deoxycholic acid there... I am not sure if deoxycholic acid composition change in the gallbladder (for example from 2% to 1%) actually lead to a major physiological change... I checked some literature, if I am correct, it seems DCA is a 'secondary bile acid' which should in theory only appear in the intestine, serum and hepatocyte? Why it appears in the gallbladder... Anyone has suggestions or explanations about this?
There are different salts are generated in form of bile acids. these are mainly deoxycholic Chenodeoxycholic acid (CDCA), cholic acid (CA), deoxycholic acid (DCA), and lithocholic acid (LCA). But in entero -hepatic circulation enzymes influence and transform and convert these acids during biotrasnformation..Different molecular forms of bile acids can be synthesized in the liver these form Gallstones;evoke Bile acid diarrhea; . Deoxycholic acid (DCA) may convert in muricholic acid; or taurine-conjugated acid, , or into chenodeoxycholic acid, check its spectra you can confirm your result.
DCA (3a,12a) is a secondary bile acid , derived from cholate (3a,7a,12a) , When entering the intestine 95% of the BA are reabsorpted again , 5% loss via feaces, and entering the liver again, where secretion to bile take place , the so called enterohepatic circulation. So your DCA is an recycled secondary bile acid
Thank you for your information Ravi and Vincent. I may go ahead to check the intra-hepatic, serum and fecal bile acid to see what happens in our mice.
Perhaps nice to read
http://www.ncbi.nlm.nih.gov/pubmed/?term=24821328
http://www.ncbi.nlm.nih.gov/pubmed/?term=24560594