Rongrong Fan

5 Questions 9 Answers 0 Followers

Questions related from Rongrong Fan

How to define super enhancers?

I find it confusing how the super enhancer can be defined practically. There has been 'standards' set to define mouse embryonic stem cell super enhancers using specific transcription factors. Is...

12 March 2017 7,191 3 View

RAW264.7 or BMDM lentiviral transduction

Has anyone succeeded in transduction the RAW or BMDM cells with lentivirus (PLKO.1) without puromycin selection? The BMDM selection could be a little bit difficult if I need large scale of cells...

13 September 2015 5,761 6 View

Does deoxycholic acid (DCA) normally appear in mice gallbladder? Is it functionally important?

I am recently analyzing the bile acid composition in our mice, and I observed about 2% deoxycholic acid there... I am not sure if deoxycholic acid composition change in the gallbladder (for...

01 April 2015 2,723 4 View

Is adenovirus a useful tool to study primary macrophage functions?

I realized there are some papers showing that adenovirus activated inflammasome in primary mice bone marrow macrophages. However, still there are some papers using adenovirus over-expression or...

08 April 2013 4,859 4 View

RAW 264.7 differentiation issue.

I have recently started to culture RAW 264.7 cells. I have never cultured this cell line before. I noticed from a number of sources that they may also be able to differentiate into M1 and M2...

26 March 2013 7,772 13 View