It is well known that layer 2/3 excitatory neurons in cortex are labeled by retrograde uptake of cholera toxin-B. My interest would be to also label layer 5 pyramidal neurons? So I would like to request if anyone has used or had experience with retrograde tracing of cholera toxin-B who could enlighten on this issue. I would appreciate your effort.
Pierre Veinante
Hi,
No problem with layer V neurons, as it is the case for most neurons. Cholera toxin B (CTb) is captured by terminals, whatever the type, as long as they contain gangliosids GM1. Find the terminal fields of the neurons you want to label and inject your CTb there. For protocol, you can take any published protocol, it's pretty straightforward.
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