I want to transfect phage T2 genome into HEK293 cells in order to produce phage particles and cell lysis. But I don't know whether the genes will work or not. What is phage biology in mammalian cells?
My challenge is that may a phage gene expresses in mammalian cell?
Hi everyone, I'm working on a project related to seismic risk analysis and I am searching for online tools that provide hazard curves and hazard disaggregation for various locations around the...
05 June 2024 851 15 View
I need TAIS ( The Attentional and Interpersonal Style Inventory) (English version), please send it to me if possible. Thanks
15 October 2023 920 1 View
Hello everyone, I am conducting research on Probabilistic Seismic Hazard Assessment (PSHA) and I am looking for software recommendations that can handle PSHA with mainshock and aftershock...
07 July 2023 9,168 1 View
I need to implement some PE with different PIR in network on chip. Are there anyone to help me? I am trying to know how it is possible in NOXIM and BookSim. But, up to now, I dont get any result..
19 June 2023 8,650 2 View
Which combination of polyol and isocyanate can be used to synthesize a stretchable (at least 100%) polyurethane sheet/film? I have tried monomeric and polymeric MDI along with Castor oil as well...
05 June 2023 8,826 2 View
Hi colleagues, I am planning to study the interaction of a DNA-binding protein with a plasmid DNA sequence by FRET assay in vivo. I want to label my plasmid with FITC and transfect it into...
19 February 2023 8,024 4 View
We have a variable load which is connected to a pv panel via a boost conveter. As depicted in the figure, the controller controls the output voltage, and doesn't grant mppt. What happens when the...
01 October 2021 2,865 1 View
It is well known that you can not help yourself by reviewing a manuscript (written by yourself) immediately. There is a need for a distance to promote the quality of reviewing. Please share your...
03 May 2021 7,796 9 View
I am curious about the applications of one of my research interests, AI, learning in the other majors. Please guide me about the applications of this field that you have encountered so far.
11 April 2021 9,840 7 View
Consider a lot number of objects each with a time series history. these objects could be clustered to groups based on their history information similariry. In each group of similar objects, the...
29 March 2021 1,976 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View