Does anyone use saturated trehalose (~75% W/V) for reverse transcription or PCR?

Ashwini Sunkavalli @Ashwini_Sunkavalli2

04 April 2015 1 698 Report

How do you make and store saturated trehalose? What do you do to dissolve it? '.

Thank you!

M N Manoj

Do not know about saturated trehalose, but we usually make it at 40% w/v for use in PCR. Why saturated solution?

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