Ashwini Sunkavalli

4 Questions 8 Answers 0 Followers

Questions related from Ashwini Sunkavalli

Which company has the best BamHI and EcoRI?

Hello Everyone, I am curious to know your experiencers from different brands for the above enzymes. Also if these are a good restriction enzyme combinations for double ligation of 3 PCR products...

01 January 2018 4,476 9 View

Has anyone run their ligation reactions on gel without heat inactivation?

Hello, If anyone has run their ligation product on gel without heat inactivation at 65C, please let me know your results and if you ran into any issues

01 January 2018 6,251 0 View

Does anyone use saturated trehalose (~75% W/V) for reverse transcription or PCR?

How do you make and store saturated trehalose? What do you do to dissolve it? '. Thank you!

04 April 2015 665 1 View

Could anyone explain how the coverage is calculated in amplcon seq?

Hi, Could anyone explain how the coverage is calculated in amplicon seq? Since a target is PCR amplified and sequenced and there is a lot of duplication and no/less unique reads. Thank you, Ash

01 January 2015 3,850 2 View