I am preparing for evaluation of canine ID's and I am wondering if I can use the same protocol for PI staining as for bull's sperms. Thank you kindly for any suggestions.
Yes you can use the exactly the same protocol for PI staining for canine sp. as discribed for bulls.
Greatings from Germany
Dear Dr. Krienke,
thank you kindly for quick response.
Best regards from Prague.
yes, it works. You could also use it associated to carboxyfluorescin or similar. Live/dead viability sperm kit works too, but they swim fast and are difficult to count. The old method (http://www.sciencedirect.com/science/article/pii/S0093691X97000666) worked fine
(I forgot that we are in the flow cytometer era... I'm getting old!)
Yes, it can be used with same protocol similar to bulls. I use 2.4mM solution most of the times. It works for both manual microscopy and flow cytometry.
We are a Nanotechnology company that has developed fertility treatments for sperm. We collect and deplete moribund sperm and enrich for viable sperm and or Gender. We have worked with a variety of species; bovine,Equine,porcine,human, canine dolphins, etc. . Let me know your interest in working with us.We are looking for partners to help optimize this technology as well as other aspects of fertility. [Fetal cell isolation and enrichment]
Henry
PI works fine in most species. I have personally used it in exotic species as well as more common domestic species. It's also useful in teaching protocols.
We now manufacture PI magnetic nano particles to deplete moribund sperm. the sperm is tagged and removed. Let me know your interest
Henry [email protected]
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