Hello everyone! I am willing to perform a phosphorylation assay, just to check if my target protein is auto-phosphorylated and if it phosphorylates another proteins found in IP.
For that case, which one is better to use Phos-tag Biotin (for WB) or Phos-tag SDS gels? I was reading these two methods and I though Phos-tag biotin would be easier, but most of the comments here refers to Phos-tag SDS-PAGE.
I appreciate any kind of help!!
http://www.wako-chem.co.jp/english/labchem/journals/phos-tag_GB2013/pdf/Phos-tag.pdf
Camila: Phos-tag SDS gel should be a sensitive method to detect phosphorylation of, as well as to detect any phosphorylated product(s) by your target protein. The phospho-protein bands on your WB gel shall show different molecular sizes in the two approaches.
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