Does anyone use Anti-HA magnetic beads for protein purification?

17 March 2014 0 1K Report

I would like to know if there is anyone who has used this method and how the result was.

Badges
Science method

More Nahid Nasir's questions See All

Why is my HPRT1 housekeeping gene not coming at same Ct in qPCR?

I am doing qPCR but my HPRT1 Ct is not coming at the same point. I see a 2 cycle spread in Ct. I have checked my RNA quality A260/280, 230/280 values, did ethidium bromide gel. I used superscript...

04 July 2024 5,605 3 View

How I can enhance my protein expression in E.coli BL21(DE3) ? How I can increase yield? How I can reduce non-specific bands on SDS-PAGE ?

I am working with leishmania threonine synthase (LdTS) enzyme of aspartic acid pathway which is ~75kDa protein. I purified the protein and confirmed by western blot too. The condition used for...

25 March 2024 7,978 3 View

How I can enhance my protein expression in E.coli BL21(DE3) ? How I can increase yield?

Hey there, currently I am working on threonine synthase enzyme of Leishmania. I have successfully cloned it into E.coli DH5alpha strain. I have also transformed it into E.coli BL21(DE3) strain. I...

02 February 2024 1,962 4 View

How I can enhance my protein expression in E.coli BL21(DE3) ? How I can increase yield?

Hey there, currently I am working on threonine synthase enzyme of Leishmania. I have successfully cloned it into E.coli DH5alpha strain. I have also transformed it into E.coli BL21(DE3) strain. I...

02 February 2024 6,431 3 View

Why there is change in mobility of RNA after RNase treatment than smear?

Hi, I extracted total cellular RNA from HEK293T cells by using a commercial kit. I left samples untreated or treated with 2ul RNaseA/T1 mixture (invitrogen) in the presence of PBS or protein...

11 January 2024 2,362 4 View

What is a Systematic Literature Review (SLR) and how to conduct a SLR?

What is a Systematic Literature Review (SLR) and mention its scientific procedure.

12 December 2023 4,877 5 View

Pepper on goji berry?

I want to find the research paper on the grafting of goji berries as rootstock.

09 December 2023 7,211 1 View

How season variation effect on Biofilm formation?

Biofilm Formation

10 November 2023 484 3 View

Has Q5 DNA polymerase ever been used in gene cloning of Leishmania?

Not been able to find any article on this. Help me to find out.

09 November 2023 1,540 1 View

Can XRD CS be bigger than TEM particle diameter?

Hi XRD and TEM experts It doesn't make sense to me but I still wanted to see if it's possible to have XRD crystallite size bigger than TEM particle diameter. I'm talking about high BET oxide...

31 October 2023 1,767 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View