recently I am working on zebrafish and I want to detect zebrafish insulin by immunostaining but I do not know which insulin antibody works on immunostaining in Zebrafish?
so I will be thankful if anyone help me this my problem.
If you cannot find a specific antibody that detects zebrafish insulin, try a polyclonal antibody, such as guinea pig.anti-human insulin antibody.
Since insulin sequence is well-conserved, it is much more likely that a polyclonal anti-insulin serum (or IgG) will have a fraction of IgG molecules which recognize shared epitopes between the two species.