3 Questions 7 Answers 0 Followers
Questions related from Amit Kumar Singh
I am using 2 million cells, adding equal volume of 2x sample buffer, boiling for 15 min at 95 degree, centrifuge and loading on gel. Please suggest me What are the changes I can do in my protocol?
14 January 2016 3,422 4 View
Why there are more dead cells are present after one week of reviving? I centrifuge the cells at low RPM and then wash with PBS and add fresh media to it but the no. of dead cells remain same? Can...
11 August 2015 8,481 3 View
Cells are dying after transfection due to toxicity of either DNA/ lipofectamine reagent What specific media should be used while diluting the lipofectamine reagent(recommended opti MEM or any...
08 July 2015 342 9 View
