Amit Kumar Singh

4 Questions 8 Answers 0 Followers

Questions related from Amit Kumar Singh

Can anyone please tell me the method of sample preparation for SDS-PAGE?

I am using 2 million cells, adding equal volume of 2x sample buffer, boiling for 15 min at 95 degree, centrifuge and loading on gel. Please suggest me What are the changes I can do in my protocol?

14 January 2016 3,469 4 View

Why are there more dead cells present after one week of reviving?

Why there are more dead cells are present after one week of reviving? I centrifuge the cells at low RPM and then wash with PBS and add fresh media to it but the no. of dead cells remain same? Can...

11 August 2015 8,529 3 View

How can I optimize the transfection efficiency using lipofectamine 3000(invitrogen)?

Cells are dying after transfection due to toxicity of either DNA/ lipofectamine reagent What specific media should be used while diluting the lipofectamine reagent(recommended opti MEM or any...

08 July 2015 436 9 View

What is the best way to preserve RNA?

What is the best way to preserve RNA -20 degree or -87 degree?

01 July 2015 5,540 0 View