I mean 'immobilized haptens' here as hapten molecules (or IgE epitopes) which are directly bound to a solid phase, not as hapten-conjugated lipid membranes like studies below.
Article Availability of dinitrophenylated lipid haptens for specific...
Dear Ryosuke,
I assume this works. Of course you need to take a hapten that is recognised by the IgE on the surface of the cells. Why do you want to bind haptens to the plate instead of using soluble haptens? I think it is more difficult to get this done as compared to see activation of the cells... :-)
Good luck and keep us informed whether it worked!
Dorrit
Hi Ryosuke,
This model can work if the steric hindrance will not be too strong
Dear Dorrit,
Thank you for your helpful comment!
I think this system would be more difficult than using hapten-conjugated carrier proteins, too.
But I also think this can work, depending on the conditions.
I'll try it.
Thank you!
Rio
Dear Sergey,
Thank you for your comment!
I'm worried about the steric hindrance, too.
Rio
Dear Ryosuke,
I think this system can work,you can select DNP-BSA as immobilized haptens
Dear Ryosuke
One option would be to mix e.g. DNP-BSA with Fibronectin to increase adhesion of RBL cells to plates. It may also increase sensitivity, although it is difficult to compare soluble phase with solid phase stimulation. Without fibronectin you will lose many of the cells if you decide to wash them.
Dear Yang,
Thank you for your comments.
I have tried several kinds of antigens to be immobilized, and they worked as expected.
Dear Franco,
Thank you for your helpful comment.
I have tried with collagen ever, but never with fibronectin.
I want to try it if there's a chance.
We have presented studies regarding effects of immobilization of antigens in the Japanese society of food chemistry.
Now I am preparing a manuscript of it to submit anywhere.
I'd like to say thanks to all of those who kindly commented on this question thread.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus