Does anyone know where to get peptide substrate sequences for the cathepsins?

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Steingrimur Stefansson

Hi. There are a bunch of companies that sell cathepsin substrates. Just be aware that most of the cathepsin substrates can also be cleaved by serine proteinases.

http://www.anaspec.com/products/productcategory.asp?id=614

http://www.mpbio.com/index.php?cPath=1_5_25_147&country=223

Marcia Moss

The reason I wanted to make our own is the Anaspec ones are in a kit and quite expensive. They don't give the substrate info. I was hoping to get fairly selective substrates for most of the cathepsins.

I dont know of any publication that has a list of cathepsin specificities. Take a look at these papers and see of you can narrow it down.

http://scholar.google.com/scholar?q=%22cathepsin%22+%22P1%22+%22P2%22+%22P1%22%22+%22P2%27%22+&btnG=&hl=en&as_sdt=0%2C21

Thank you for the links