How to over come the error in GROMACS simulation (Protein-Ligand) during Add-Ions ?

HI All, "ERROR : No.of Coordinates in Solv.gro and Topol.top not matching" I am trying to run GROMACS simulation for Protein-Ligand complex, and I am facing this issue, and I dont know how to...

31 December 2019 8,943 3 View

How to construct BH plot, Jobs plot for UV absorbance data ?

I have data for UV absorption of anionic sensors. I want to construct BH plot and Jobs plot to find the binding ratio for sensor-ligand complex.

31 December 2016 6,228 4 View

Suggest some Peer Reviewed Journals "Without Processing Fee" ?

Hi Friends, I am searching for some peer reviewed journals, that do not require any processing or publication fee. Can anyone suggest some well known journals. It has to be Indexed in...

04 May 2016 2,899 8 View

How can I identify a Species of a Streptomyces strain?

One of my 16S rRNA gene sequencing resulted in 93% similarity to NCBI database., suggesting it could be a novel or new species., which im not sure yet. For this case, what should i further do...

08 September 2015 7,275 11 View

Does chloroform melt the Eppendorf tube ?

01 February 2015 9,907 9 View

What is the UV range for Magnesium nanoparticle ?

I have been searching for a while now., but I couldn't get any information on the spectrum range for Magnesium nanoparticles. For.eg., Silver Nanoparticle falls between 400 to 500nm in UV...

10 November 2014 6,525 4 View

Does Freezing affect the result of Histopathology analysis?

If a tissue sample is stored in Deep Freezer (-20 C) for a few days, and then thawed to room temperature before Histopathology analysis, will it affect the result?

05 June 2014 5,123 4 View

What Protein-Ligand Docking tools are available to use?

I have been trying to do some Docking studies. I have tried running Auto-Dock in my laptop, for some reason, it wouldn't work with my laptop. I works fine on my friends laptop,just not in mine....

05 June 2014 1,264 2 View

Can anyone give me a protocol for a biochemical assay for a tissue sample?

I am looking for laboratory biochemical assays for tissue samples to estimate Catalase, Glutathione Peroxidase, Superoxide Dismutase in an animal tissue sample. Alternative to Gel Activity...

05 June 2014 166 12 View

Anyone interested in working on Plasmodium BioInformatics ?

I am looking for a research scholar (PhD student / Professor / JRF) who is interested in working on Plasmodium BioInformatics. I do have a concept to work with and also open to new topics, if you...

04 May 2014 2,249 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View