ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

Why am I getting western bands with white bubbles?

12 October 2020 6,976 3 View

How would I determine if a protein (PD-1) is necessary for establishment of latent infection by a virus in a CD4 memory cell line?

If I were to plan an experiment to deduce whether or not PD-1 is absolutely necessary for HIV to establish latent infection in CD4 memory cells, how would I go about doing this? More...

09 March 2020 7,774 2 View

What is the most appropriate statistical analysis to assess between and within subject differences over time with most individuals with 2 time points?

I am looking at how hormone concentrations change over time both between individuals and in relation to fixed effects of predictor variables (n=7) within individuals. I have a large dataset of...

02 July 2019 8,734 9 View

I want to selecte resistance antibiotic bateria in the pond ? I was offer the MHA already but Could you give me some idea about an other medium ?

The antibiotic target is SMX and OCT,

30 June 2019 5,542 5 View

Calculating beam nodal displacements/rotations using SAP2000 and programming system matrices in MATLAB - Differences in responses?

I have programmed an Euler-Bernoulli beam, supported on springs, using both SAP2000, and by using the stiffness matrix method in MATLAB. The nodal (joint) displacements calculated due to an...

13 March 2019 8,597 3 View

Caco-2 cells showing high TEER (2000ohm cm2) after 21 days growth, is this normal?

I am working on the Caco-2 cell line, I'm particularly interested in changes in permeability under inflammatory stimulation. I am using growth conditions: 12-well transwell plates, seeding...

14 January 2019 3,047 2 View

Why Aren't My 1L Bacterial Cultures Growing Properly?

I performed a fresh transformation (with sequenced plasmid) into commercial BL21 competent cells. I scrape a colony into a starter culture w/ antibiotic overnight. I check the O.D. of the starter...

15 October 2018 3,171 9 View

How does emulsion polymerization work?

I would like a simple explanation of emulsion polymerization, and I would like to know whether the process requires UV light and how UV light will effect the process.

15 March 2018 7,706 3 View

Does Abcam's anti-TrkB antibody (ab18987) also bind activated or phosphorylated TrkB (p-TrkB)?

I've been using this anti-TrkB antibody in studies involving the activation of TrkB by BDNF. When I called Abcam to ask if it also binds p-TrkB, I was told that they've never tested it, and have...

20 February 2018 2,758 3 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

What is difference between In-line transmission (%) and Total transmission (%) value of optically transparent Ceramics?

I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...

20 February 2021 9,405 6 View