Hi, in a few months I will have to do a comet assay by taking blood sample from people as a sample. The problem is that I don't know anything about it, so I wonder if there is someone who can help me and tell in which book I can find information and protocols about this.
If you have single gel electrophoresis kit and comet apparatus for visualization, you can follow these standardized protocols.
SinghNP,McCoyMT,TiceRR,SchneiderEL.AsimpletechniqueforquantitationoflowlevelsofDNAdamageinindividualcells.ExpCellRes.1988:175:184–191.
MaheshwariN,MahmoodR.3,4-Dihydroxybenzaldehydeattenuatespentachlorophenol-inducedcytotoxicity,DNAdamageandcollapseofmitochondrialmembranepotentialinisolatedhumanbloodcells.DrugChemToxicol.2020:28:1–18.
The comet assay, also known as single-cell gel electrophoresis, is a sensitive and widely used technique for assessing DNA damage in individual cells. It involves embedding cells in agarose gel on a microscope slide, lysing the cells, and subjecting the DNA to electrophoresis. The resulting "comet-like" image reveals the extent of DNA damage based on the migration of fragmented DNA.
If you're looking to learn about the comet assay and its protocols, there are several resources you can refer to. While books can provide comprehensive information, you may find it more convenient to access online resources, which often offer updated protocols and procedures. Here are a few suggestions for learning about the comet assay:
Remember to consider the publication dates and ensure the information is up to date, as protocols and techniques can evolve over time. It may also be helpful to consult with experts in the field or collaborate with experienced researchers who have prior experience with the comet assay.
Good luck with your research and learning journey!
These video playlists might be helpful to you:
https://www.youtube.com/playlist?list=PLzBiAPKi8vOPbE7Db9mKwloe34E8rXXEU
https://www.youtube.com/playlist?list=PLzBiAPKi8vONSqjcweFWHMRy13y3484bE
https://www.youtube.com/playlist?list=PLzBiAPKi8vOMheKwb0mu1dcZRaeGUFSlw
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