Does anyone know what non-specific black dots are in Von Kossa staining and how to get ride of them?

More Mengistu Gebere's questions See All

What is the recommended proportion of adaptors and non-adaptors of agricultural Technolgies adoption?

Many papers use a larger proportion of non-adopters as compared to adopters for adoption studies. However, there is no common logic among these papers in the proportion of adoptors and...

23 January 2024 7,957 0 View

Which test programs could be developed to come to an enhanced understanding of the impact-dynamic mechanical properties of Metal Lattice Structure?

Additively Manufactured Metal Lattice Structures

10 January 2023 8,372 1 View

Is there any place to download regional CMIP6 data?

I want to get the CMIP6 data set for the eastern Africa region but I couldn't get a way to approach it. Is there anyone with such information?

06 July 2022 137 3 View

Which techniques of methane emission measurements are commonly applicable for developing world?

In developing countries, highly sophisticated technologies for methane emission might be expensive. I am seeking technical advice on easily applicable and efficient techniques.

04 July 2022 5,338 0 View

Do you think that education always have positive impact on economic development?

I perceived that rural community members with ample experience tend to participate on various agricultural development activities than educated one (who are searching for other employment...

24 June 2022 1,663 11 View

How can I draw graphs of generalized petersen graph?

I am doing research on generalized Petersen graph. but I can't draw its graph on Latex, please give me hint on the package and commands to draw this graph on Latex.

16 March 2022 5,031 4 View

Can I use Wilcoxon signed rank test, to test if there is seasonal variation in abundance between seasons?

I have species abundance (different sp.) data collected from 6 sites at two different seasons. I want to test if there is seasonal variation in the bundance of species by using the Wilcoxon...

20 February 2022 5,278 5 View

Which CMIP6 models are suited to use in Eastern African climate change projection? Is there any suggestion in selecting appropriate models?

I am interested in working climate projection and the probable impact of climate change on water resources in Eastern Africa. Which CMIP6 models are suited to use in Eastern African climate change...

09 December 2021 8,368 3 View

Is there any free climate modeling software?

I am going to use climate models in working climate change prediction. I want to get if there are software other than EdGCM.

01 June 2021 4,044 6 View

Do you know sustainable and efficient method of monkey population controlling techniques, methods and technologies?

The population of monkeys is increasing rapidly in zege church forest which causes the production of fruits and vegetables fruitless for the community living in the area. The community entirely...

26 March 2020 7,887 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View